CETSA
Cellular Thermal Shift Assay

Publications

Our Publications

1.Liang YY, Bacanu S, Sreekumar L, Ramos AD, Dai L, Michaelis M, Cinatl J, Seiki T, Cao Y, Coffill CR, Lane DP, Prabhu N, Nordlund P (2021). CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug toxicity. Cell Chem Biol, 2021 S2451-9456(21)00306-8.

2. Sun J, Prabhu N, Tang J, Yang F, Jia L , Xiao K, Tam WL, Nordlund P, Dai L (2021). Recent advances in proteome-wide free target deconvolution for bioactive small molecules. Med Res Rev, 21788 (Online ahead of print)

3. Prabhu N, Dai L, and Nordlund P (2020). CETSA in integrated proteomics studies of cellular processes Curr Opi Chem Biol, Feb;54:54-62

4. Dziekan JM, Wirjanata G, Dai L, Go KD, Yu H, Lim YT, Chen L, Wang LC, Puspita B, Prabhu N, Sobota RM, Nordlund P, Bozdech Z (2020). Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome Nature Protocols, 15(6):1881-1921

5. Langebäck A, Bacanu S, Laursen H, Mout L, Seki T, Erkens-Schulze S, Ramos AD, Berggren A, Cao Y, Hartman J, van Weerden W, Bergh J, Nordlund P, Lööf S (2019). CETSA based target engagement of taxanes as biomarkers for efficacy and resistance. Sci Rep 2019 12;9(1):19384

 6.     Dai L, Prabhu N, Liang YY, Bacanu S, Ramos A, Nordlund P. Horizontal Cell Biology: Monitoring global changes of protein interaction states in cells with proteome-wide CETSA. Annual Reviews of Biochemistry, in press, 2019

7.     Sun W, Dai L, Yu H, Puspita B, Zhao T, Li F, Tan JL, Lim YT, Chen MW, Sobota RM, Tenen DG, Prabhu N, Nordlund P. Monitoring structural modulation of redox-sensitive proteins in cells with MS-CETSA. Redox Biol. 2019 Mar 14;24:101168. doi: 10.1016/j.redox.2019.101168. [Epub ahead of print]

 

8.     Dziekan JM, Yu H, Chen D, Dai L, Wirjanata G, Larsson A, Prabhu N, Sobota RM, Bozdech Z, Nordlund P. 2019 Identifying purine nucleoside phosphorylase as the target of quinine using cellular thermal shift assay. Sci Transl Med. Jan 2;11(473).

 

9.     Lim YT, Prabhu N, Dai L, Go KD, Chen D, Sreekumar L, Egeblad L, Eriksson S, Chen L, Veerappan S, Teo HL, Tan CSH, Lengqvist J, Larsson A, Sobota RM, Nordlund P. 2018 An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactions. PLoS One. Dec 6;13(12)

 

10.     Dai L, Zhao T, Bisteau X, Sun W, Prabhu N, Lim YT1, Sobota RM, Kaldis P, Nordlund P. Modulation of protein interaction states through the cell cycle. Cell. 2018 173(6):1481-1494

 

11.     Tan CSH, Go KD, Bisteau X, Dai L, Yong CH, Prabhu N, Ozturk MB, Lim YT,Sreekumar L, Lengqvist J, Tergaonkar V, Kaldis P, Sobota RM, Nordlund P. Thermal proximity coaggregation for system-wide profiling of protein complex dynamics in cells. Science. 2018 10.1126/science.aan0346. 

 

12.     Kitagawa M, Liao PJ, Lee KH, Wong J, Shang SC, Minami N, Sampetrean O, Saya H, Dai L, Prabhu N, Diam GK, Sobota R, Larsson A, Nordlund P, McCormick F, Ghosh S, Epstein DM, Dymock BW, Lee SH. Dual blockade of the lipid kinase PIP4Ks and mitotic pathways leads to cancer-selective lethality. Nature Commun. 2017;8(1):2200. 

 

13.     Almqvist H, Axelsson H, Jafari R, Dan C, Mateus A, Haraldsson M, Larsson A, Martinez Molina D, Artursson P, Lundbäck T, Nordlund P. CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil. Nature Commun. 2016 Mar 24;7:11040. 

 

14.     Martinez Molina D, Nordlund P. The Cellular Thermal Shift Assay: A Novel Biophysical Assay for In Situ Drug Target Engagement and Mechanistic Biomarker Studies. Annual Rev Pharmacol Toxicol. 2016 6;56:141-61. 

 

15.  Jafari R, Almqvist H, Axelsson H, Ignatushchenko M, Lundbäck T, Nordlund P, Martinez Molina D. The cellular thermal shift assay for evaluating drug target interactions in cells. Nature Protoc 2014;9(9):2100-22. 

 

16.  Savitski MM, Reinhard FB, Franken H, Werner T, Savitski MF, Eberhard D, Martinez Molina D, Jafari R, Dovega RB, Klaeger S, Kuster B, Nordlund P, Bantscheff M, Drewes G. Tracking cancer drugs in living cells by thermal profiling of the proteome. Science. 2014;346(6205):1255784.

 

17.  Asial I, Cheng YX, Engman H, Dollhopf M, Wu B, Nordlund P, Cornvik T. Engineering protein thermostability using a generic activity-independent biophysical screen inside the cell. Nature Comm. 2013;4:290.

 

18.  Martinez Molina D, Jafari R, Ignatushchenko M, Seki T, Larsson EA, Dan C, Sreekumar L, Cao Y and P Nordlund Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay. Science, 2013;341(6141):84-7.